MtoZ Biolabs



peptide mapping

Peptide mapping is a widely used approach for analyzing biopharmaceuticals, including antibody, protein and peptides. The common peptide mapping process only uses trypsin for protein digestion, and the average coverage rate of identified protein is about 60%. In order to obtain the full sequence information of the targeted protein, MtoZ Biolabs uses up to 6 kinds of proteases for protein digestion (Trypsin, Chymotrypsin, Asp N, Glu C, Lys C and Lys N). After protein digestion, sequences of peptide are mapped for full length sequence of biopharmaceuticals. MtoZ Biolabs has also developed LC MS MS based peptide mapping technology, ensuring accurate peptide mapping analysis. This technology can be used to analyze the sequence of biopharmaceuticals and confirm complete expression of recombinant protein. With well established technology and professional expertise, we can guarantee 100% peptide coverage and accurate data analysis.

n and c terminal sequencing of proteins

N and C terminal sequences are important structures and functional parts of protein and polypeptide, and they may play decisive roles in the biological function of protein. The main methods for protein C terminal sequencing include carboxypeptidase, chemical and tandem mass spectrometry, and the common methods for N terminal sequencing include Edman degradation sequencing and mass spectrometry. Each of these methods has the respective advantage and shortcoming. Therefore, the combination of a variety of different sequencing methods can adapt to the requirements of multiple protein sequencing. For example, Edman degradation may not well solve the problem of blockage and protein modification on the N terminal end. This drawback can be overcomed by mass spectrometry analysis. MtoZ Biolabs uses both Edman degradation system and MALDI TOF for N or C sequencing of biopharmaceutical products.

molecular weight analysis

At present, there are two types of mass spectrometry used for molecular weight analysis: Matrix assisted laser desorption ionization (MALDI TOF) and Electrospray ionization (ESI MS). The two methods have their own advantages and applications. MALDI TOF can be used to directly measure the molecular weight of biopharmaceuticals, while ESI MS analyzes multiple charge signals obtained by electric spray, and speculates the precise molecular weight through deconvolution. MALDI TOF MS is more suitable for analyzing biopharmaceuticals with molecular weight less than 25kDa. ESI MS is recommended for molecular weight identification of protein substances with molecular weight of larger than 25kDa. MtoZ Biolabs has established a platform with both MALDI TOF and ESI MS technologies, which can be used to determine the molecular weight of all kinds of biopharmaceuticals, including antibodies, vaccines, proteins drugs, and polypeptides.

acyl coAs analysis

MtoZ Biolabs offers targeted acyl coAs analysis service using ACQUITY UPLC TripleQuad5500 (Waters AB Sciex), which has characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of acyl coAs even in low abundance.

targeted metabolomics

Targeted metabolomics is a study in which defined metabolites in a sample are identified and quantitatively analyzed. Quantitation and semi-quantitation of defined metabolites are undertaken through the use of internal standard compounds. Targeted metabolomics has an advantage of high specificity and accuracy. Thus, this method has been widely used to analyze and compare multiple targeted metabolites under different physiological states, and is a critical analytical method for discovery of new biomarker in metabolic diseases and study of early diagnosis of diseases. MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms, which have characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of targeted metabolites, even in low abundance.